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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
shh
|
|
laevis
|
NF stage 50
|
brain
,
neural tube
,
zona limitans intrathalamica
,
thalamus
|
rspo2
|
|
laevis
|
NF stage 11
|
ectoderm
,
ventro-lateral marginal zone
,
superficial
,
deep
|
rspo2
|
|
laevis
|
NF stage 29 and 30
|
brain
,
diencephalon
,
midbrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
rspo2
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
ventro-lateral marginal zone
,
ventro-lateral
,
anterior neural ridge
|
rspo2
|
|
laevis
|
NF stage 50
|
brain
,
diencephalon
,
zona limitans intrathalamica
|
rspo3
|
|
tropicalis
|
NF stage 14
|
pre-chordal neural plate
,
mesoderm
,
circumblastoporal collar
,
pre-chordal neural plate border
|
rspo3
|
|
tropicalis
|
NF stage 22
to
NF stage 44
|
branchial arch
,
tail bud
,
central nervous system
|
|
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Figure 2. Expression Analysis of Xenopus and Mouse R-spondins(A) Rspo2 expression during Xenopus development at the indicated embryonic stages analyzed by RT-PCR. Histone H4 was used for normalization. −RT, minus reverse transcription control.(B–H) Xenopus whole-mount in situ hybridizations of the indicated genes.(B) Stage 11 embryo, dorso-vegetal view; dbl, dorsal blastoporal lip.(C) Stage 12 embryo, dorsal view with anterior up. An anterior neural expression domain is indicated by arrowhead.(D) Stage 15 embryo, dorsal view with anterior up.(E) Stage 14 embryo, dorsal view with anterior up.(F and G) Tailbud stage embryos; ba, branchial arches; cm, cranial musculature; di, diencephalon; dnt, dorsal neural tube; mhb, midbrain-hindbrain boundary; ov, otic vesicle; pn, pronephros; pdm, proctodeum; s, somites; tb, tailbud mesoderm. Inset in (F) shows a transverse section at the level indicated by arrowhead, showing expression in dorsal neural tube and in the dorsal- and ventral-most parts of the somites.(H) Dissected Xenopus tadpole brain (lateral view) showing expression in diencephalons (di) and zona limitans intrathalamica (zli), where sonic hedgehog is expressed (inset). dt, vt, dorsal and ventral thalamus, respectively; sc, spinal cord; tel, telencephalon.(I–M) Mouse whole-mount in situ hybridizations of the indicated genes.(I) Limb buds of day 12.5 mouse embryos. AER, apical ectodermal ridge.(J) Day 7.5 mouse embryo showing Rspo3 expression in the primitive streak.(K–M) Day 9.5 mouse embryos. di, diencephalon; dnt, dorsal neural tube; met, metencephalon; tel, telencephalon. |
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Display additional annotations [+]
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Figure 3. Regulation of Xenopus R-spondins by Wnt Signaling(A) Comparison of XRspo2, XWnt8, and XWnt3A expression pattern in early neurula embryos by whole-mount in situ hybridization. Dorsal view, anterior up.(B) Top: Diagram of experiment. 4-cell stage embryos were injected with 50 pg pCS-ppl (preprolactin), pCS-XWnt8, or pCS-β-catenin into each blastomere, and DMZs were dissected, cultured until stage 11 equivalent, and analyzed by RT-PCR. Bottom: RT-PCR analysis of the indicated genes. −RT, minus reverse transcription control.(C–E) 4-cell stage embryos were injected with 50 pg pCS-Wnt8, pCS-Wnt3A, or pCS-ppl into one blastomere and fixed at stage 11 for in situ hybridization with XRspo2. |
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rspo2 (R-spondin 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 15, dorsal view, anterior up. |