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Figure 1. Expression of Xenopus Sall1, Sall3, and Sall4 during hindlimb regeneration at the regeneration-complete stage and the regeneration-incomplete stage. A: Top; whole-mount in situ hybridization (WMISH) of Sall4 in stage-53 and -57 limbs and in stage-53 and -57 regenerating hindlimbs at 0, 1, 3, and 5dPA. Bottom; RT-PCR comparison of Sall1, Sall3, and Sall4 expression patterns in stage-53 and -57 regenerating hindlimb blastemas and pseudoblastemas at 0, 1, 3, and 5dPA. Amputation was through the mid-zeugopodia and blastemas (from stage-53 limbs) or pseudoblastemas (from stage-57 limbs) were collected for analysis at 0, 1, 3, and 5dPA. At 0dPA about 1 to 2 mm of tissue from the distal end of the amputated limb was used as the internal tissue control. Odc (ornithine decarboxylase) was used as an internal control. WMISH data was taken from Neff et al. (2005) with the authors' permission. B: QPCR comparison of Sall1, Sall3, and Sall4 expression patterns in stage-53 and -57 regenerating hindlimb blastemas and pseudoblastemas at 0, 1, 3, and 5dPA. Sall gene expression levels are normalized to odc and then shown relative to stage-53 0dPA levels of expression, which is arbitrarily set to 1.0. RT-PCR and QPCR were performed as described in King et al. (2009). |
