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Gene/CloneSpeciesStageAnatomy ItemExperimenter
smad9xenopus presumptive ectoderm [+] 

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Experiment details for smad9

Cibois M et al. (2015) Assay

BMP signalling controls the construction of vertebrate mucociliary epithelia.

Gene Clone Species Stages Anatomy
smad9.L laevis NF stage 9 presumptive ectoderm
smad9.L laevis NF stage 12 non-neural ectoderm
smad9.L laevis NF stage 12 non-neural ectoderm
smad9.L laevis NF stage 15 epidermis , non-neural ectoderm
smad9.L laevis NF stage 15 epidermis , non-neural ectoderm , epidermal cell
smad9.L laevis NF stage 15 epidermis , ionocyte , non-neural ectoderm , epidermal cell , mucociliary epithelium
smad9.L laevis NF stage 15 epidermis , non-neural ectoderm , epidermal cell , mucociliary epithelium , ciliated epidermal cell
smad9.L laevis NF stage 17 epidermis , non-neural ectoderm , epidermal cell
smad9.L laevis NF stage 17 epidermis , ionocyte , non-neural ectoderm , epidermal cell , mucociliary epithelium
smad9.L laevis NF stage 17 epidermis , non-neural ectoderm , epidermal cell , mucociliary epithelium , ciliated epidermal cell
smad9.L laevis NF stage 19 epidermis , ionocyte , non-neural ectoderm , epidermal cell , mucociliary epithelium
smad9.L laevis NF stage 19 epidermis , non-neural ectoderm , epidermal cell , mucociliary epithelium , ciliated epidermal cell
smad9.L laevis NF stage 19 epidermis , non-neural ectoderm
smad9.L laevis NF stage 25 epidermis , epidermis inner layer , non-neural ectoderm , epidermal cell
smad9.L laevis NF stage 25 epidermis , ionocyte , non-neural ectoderm , epidermal cell , epidermis outer layer
smad9.L laevis NF stage 25 epidermis , non-neural ectoderm , epidermal cell , epidermis outer layer , mucociliary epithelium , [+]
smad9.L laevis NF stage 32 epidermis , epidermis inner layer , epidermal cell
smad9.L laevis NF stage 32 epidermis , ionocyte , epidermal cell , epidermis outer layer , mucociliary epithelium
smad9.L laevis NF stage 32 epidermis , epidermal cell , epidermis outer layer , mucociliary epithelium , ciliated epidermal cell

Display additional annotations [+]
  Fig. 1. BMP pathway activity in the developing Xenopus epidermis. (A) Immunostaining of cryosectioned Xenopus embryos revealed that the pSmad1/5/8 signal (red) colocalises with nuclear DAPI staining (blue) in both inner and outer layer cells. (B) (Left) In embryos injected with linearised plasmid DNA for the MCC reporter α-tub::GFP (50 pg/embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Middle) In embryos injected with linearised plasmid DNA for the ionocyte reporter pendrin::GFP (25 pg/ embryo), the pSmad1/5/8 signal (red) colocalised with GFP immunostaining (green). (Right) The pSmad1/5/8 signal (red) colocalised with P63 protein expression (white). For each marker and stage, a representative cell (yellow arrowhead) is displayed at higher magnification to show the double staining. The white dashed and dotted lines respectively mark the apical surface of the ectodermal outer layer and the boundary between the outer and the inner ectodermal layers.