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Fig. 4. Developmental expression of XBaf57. (A) RT-PCR analysis of total embryo RNA prepared at the indicted stages. XBaf57 transcripts are detected maternally and throughout gastrula, neurula and tadpole stages. RT- is a negative control without reverse transcriptase during cDNA synthesis. Histone was used as loading control. (B–I) Whole mount in situ hybridization analysis of XBaf57. (B) Lateral view and (C) sagittal section of a stage 10.5 embryo showing XBaf57 expression in the ectoderm and marginal zone but not the vegetal pole. (D) Vegetal view and (E) sagittal section of a stage 12 embryo confirming the ectodermal expression of XBaf57. The black arrowheads indicate the position of the dorsal blastopore lip. (F) Dorsal view of a stage 14 embryo showing expression in the neural plate and border between the neural plate and epidermis. (G) Dorsal view of a stage 24 embryo. XBaf57 is expressed along the central nervous system and eye. (H) Lateral view of a stage 30 embryo showing expression in the branchial arches, otic vesicle, tailbud and vascular structures such as the intersomitic vessels and posterior cardinal vein. (I) Lateral view of a stage 30 cleared embryo reveals weaker expression in the vitelline vascular network. In (F–I) anterior is to the left. |
