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FIGURE 1. Expression of XSMOC-1 during embryogenesis. A, RT-PCR analysis of XSMOC-1 expression at different stages of development. XSMOC-1 was not detectable until after stage 12. Histone H4 is shown as positive control, and ﰈRT indicates RT-PCR without reverse transcriptase as negative control. B, whole mount hybridization in situ analysis of XSMOC-1 (anterior to the left). B, ventral view of a stage 12.5 embryo showing anterior staining. C, ventro-lateral view of a stage 15 embryo showing anterior and lateral staining. Shown are lateral (D) and dorsal (E) view of a stage 17 embryo showing staining lateral to the neural plate. F, lateral views showing XSMOC-1 expression throughout the developing pronephros at stage 20 (F), stage 22 (G), and stage 24 (H). At stage 22 (G), additional expression was observed dorsal to the cement gland (arrowhead), and from stage 25 (H) onward, XSMOC-1 was expressed in the ventral region of the developing eye. I and J, dorsal views of H, following prolonged color development, showed expression in the mesencephalon, rhomben- cephalon (white arrows), and migrating neural crest (black arrows). K, transverse sections through the embryos shown in I and J in the region of the forebrain (K), hindbrain (L), and anterior trunk (M). Staining was prominent in the ventral aspect of the developing eye (K) and in the lateral regions of the hindbrain (L). Within the trunk (M), staining was observed in the pronephros and in subepithelial migrating neural crest cells. E, eye; FB, forebrain; HB, hindbrain; N, notochord; NC, neural crest; NT, neural tube; S, somite.
"This research was originally published THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL.284(28) pp.18994-19005, July 10, 2009. Copyright: The American Society for Biochemistry and Molecular Biology." |
