| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| snai1 | xenopus | blastomere [+] |
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Experiment details for snai1
Aybar MJ et al. (2003) AssaySnail precedes slug in the genetic cascade required for the specification and migration of the Xenopus neural crest.
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| Fig. 6. Inhibition of Snail activity blocks the expression of neural crest markers. One blastomere of a 2-cell stage embryo was injected with 700 pg of the different dominant negative constructs, treated with dexamethasone at stage 12.5, fixed at stage 19, and the expression of the neural crest markers analysed. The injected side is indicated by an arrowhead. (A-D) XsnailZnFGR: dominant-negative of the Snail zinc fingers. Note that the dominant negative construct inhibited the expression of all the neural crest markers analysed. (E,F) Rescue of XsnailZnFGR by XsnailGR: both mRNAs were injected in equivalent amounts and analysed as previously described. Note the normal expression of the neural crest markers in the injected side. (G,H) SnailN-GR: dominant-negative using the Snail N-terminal domain. Note that the dominant-negative constructs inhibited the expression of all the neural crest markers analysed. (I,J) Rescue of XsnailNGR by XsnailGR: both mRNAs were injected in equivalent amounts and analysed as previously described. Note the normal expression of the neural crest markers in the injected side. | |||||||||||
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| Fig. 8. Snail functions as a transcriptional repressor. One blastomere of a two-cell stage embryo was injected with 700 pg of a Snail repressor construct (A-D) or the Snail activator construct (E-H), treated with dexamethasone at stage 12.5, fixed at stage 19, and the expression of neural crest markers analysed. Arrowhead, injected side. Note that the Snail repressor construct (XsnailZnF-GR-EnR) produced an expansion of the neural crest markers on the injected side (A-D), while the Snail activator lead to an inhibition in the expression of the markers (E-H). |