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Fig. 4. Spatio-temporal expression pattern of sox21. (A) RT-PCR analysis of embryos from oocyte to stage 36 as indicated across the top. ODC was used as the loading control. (B,C,F,J,N,P) In situ hybridization of embryos stained for sox21 at indicated stages and (F-M, O) for regional brain markers as indicated. (B,C, J-M, inset P) Anterior views. (N-P) Dorsal views. (F-I) Lateral views. (D,E) Transverse sections generated from a stage 31 embryo. Symbols: Black asterisks, midbrain-hindbrain boundary; f, forebrain; h, hindbrain; m, midbrain; hy, hypothalamus; n, notochord; op, olfactory placode; ov, otic vesicle. |
