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Figure 5. Sox9 is expressed throughout the otic vesicle and in the developing semicircular canals. Developmental expression of Sox9 in the otocyst of wild type embryos by whole-mount in situ hybridization (A,B,D) and by in situ hybridization on section (C,E-I). The embryonic stage (Nieuwkoop and Faber,[1967]) is indicated in the upper right corner of each panel. A-H: Sox9 is initially expressed throughout the entire otic epithelium (A-C) and then become progressively restricted to the dorsal and ventral aspects of the otic vesicle (D-H). I: At stage 45, Sox9 is also detected in the protrusions of the developing semicircular canals (arrow). In all panels, dorsal to top. For whole embryos staining anterior is to the right. Scale bars = 300 mu m in A,B,D; 50 mu m in C,E.I. |
