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Fig. S4. Perturbation of S/Hh signaling only affects the floor plate of the brain.
Lateral views of tailbud stage control (A, D), cylopamine-treated (B, E), and Shh mRNA injected (C, F) embryos, processed by ISH for F-spondin (A–C) and FoxA2 (D–F) and stained for notochord (Tor70, brown, A–C). Loss of Hh signaling results in reduced (E) or broken (B) staining of floor plate markers in the brain in 48% of cyclopamine-treated embryos (N = 149). Shh mRNA does not cause dorsal expansion of floor plate except in the brain (arrows, C and F). Pink in C, F is staining for co-injected lineage tracer. |
