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Fig. 7. SRp38 is induced by neuroD. (A) Dorsal views of SRp38 expression are pictured at stage 21. Left: control. Right: injection of 250 pg of neuroD into one cell at two cell-stage induces robust expression of SRp38. (B) RT-PCR on ectodermal explants. Injection of 250 pg of neuroD (+N) induces expression of SRp38. EF1α is a loading control and muscle actin (MA) controls for mesodermal contamination. (C) SRp38 northern blot on total RNA in embryos overexpressing increasing doses of neuroD (125 pg to 500 pg) results in increasing amounts of SRp38 RNA. (D) SRp38 is itself regulated by neuroD, suggesting it is a component of a neuroD-induced mechanism serving to limit neurogenesis. |