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Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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sst.1 | xenopus | embryonic structure |
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Experiment details for sst.1
Oropez D and Horb M (2012) AssayTransient expression of Ngn3 in Xenopus endoderm promotes early and ectopic development of pancreatic beta and delta cells.
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Figure 1. Differential effects of Ngn3-GR temporal activation. Whole mount in situ hybridization of Stage 44 whole guts from embryos injected with 1,800 pg of XenopusNgn3-GR mRNA at the eight-cell stage. (a,d,g) Control embryos injected with Ngn3-GR mRNA but not treated with dex. (b,e,h) Dex treatment from Stages 12 to 44. Increased expression of glucagon (n = 14/20), insulin (n = 23/23), and somatostatin (n = 10/10). Arrowheads indicate ectopic insulin expression in the liver, stomach, and duodenum. (c,f,i) Dex treatment for 4 h from Stages 12 to 15. Increased insulin (n = 55/55) and somatostatin (n = 22/22) but not glucagon (n = 24/24). (j,k) Schematics illustrating organs in the whole gut of panels a and b (blue-insulin). (l) Insulin expression was detected in rare instances (n = 2/50) in posterior areas of the intestine. Pa: pancreas. | |||||||||||
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Figure 3. Transient activation of mouse Ngn3-GR and human Ngn3-GR also promoted increased insulin expression. (a) Embryos injected with 15 pg of mouse Ngn3-GR and activated with dexamethasone for 4 h at Stage 12 showed increased insulin (n = 10/10) and somatostatin (n = 7/7) expression. (e,f) Injection of 1,800 pg of human Ngn3-GR also caused increased insulin expression (n = 8/8). Pancreas is outlined in panels (a) and (c). |