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Supplementary Fig. S1. In situ hybridization of sybu expression in (A) oocytes (stages I-IV upper row; stages V-VI lower row), (B) 4-cell embryos, and (C) gastrulae. Vegetal views.
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Fig. 1.
Germ plasm enrichment of Sybu mRNA and protein in oocytes and early embryos. (A-D) Representative images of whole-mount in situ hybridization on stage VI oocytes comparing expression of (A) trim36, (B) wnt11b, (C) pgat and (D) sybu. Vegetal views are shown. (E-J) Localization of Sybu-GFP to germ plasm. (E-G) Overlap of Sybu-GFP with clustered mitochondria (mito., MitoTracker) in pricked eggs 60-min post-activation (p.a.) and at 240-min post-activation (p.a.). Scale bars represent 7.5 µm. |
