| |
Fig. 2. Expression pattern of
xOMP25 mRNA. (A) The temporal
expression pattern of xOMP25
was determined by reverse
transcription-polymerase chain
reaction (RT-PCR) analysis.
XOMP25 mRNA exists maternally
and expression is sustained until
the tadpole stage. (B�F) The
spatial expression pattern of
xOMP25 mRNA is shown by
whole-mount in situ hybridization.
Signals are observed in the
animal pole at the 8 cell stage
(B), in the anterior neural fold at
the stage 18 (C,D), and in eye,
otic vesicle, branchial arch and
neural tube in stages 28 and 35
(E,F). xOMP25 expression in the
otic vesicle and neural tube was
also seen in transverse section
(G). Anterior upward view (C). |
