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Figure S8. Expression of Xfli1 and R-spondin 3 (Rspo3) in Wnt4-depleted embryos. X. laevis embryos were injected with 12 ng Co MO (A, C, E) or Wnt4 MO (B, D, F) at the 4-cell stage in the dorsal and ventral marginal zones and cultured until stage 29-30. WISH analysis was performed for the hematopoietic marker SCL (A, B), the endothelial marker Xfli-1 (C, D) and for Rspo3 (E, F). In addition to SCL, which was strongly decreased in Wnt4 morphants, also Xfli-1 and Rspo3 were slightly reduced in the region bordering the VBI. The ratio in the right lower corner indicates the number of embryos displaying the phenotype shown in the picture. |
