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Fig 5. Concomitant localization of Rdd3 protein with blood vessel formation. Whole-mount in situ hybridization (A) and whole-mount immunostaining (B) analyses were performed to show tie-2 expression and Rdd protein localization in tailbud (st. 32) embryos. Embryos were injected with vascular endothelial growth factor Morpholino oligo (VEGF MO) (9.2 pmol/embryo) (d-f ) or H2O (a-c) in the marginal zone at the 4-cell stage. (A) In the control embryo, tie-2 was expressed in the vitelline vein (b, arrowheads), intermediate mesoderm (c, white arrowheads) and branchial arches (c, arrowheads). Localization of tie-2 expression was lost in the VEGF MO-injected embryo (d-f ). (B) Rdd protein was detected in the vitelline vein (b, arrowheads), intersomite region (c, white arrowheads), and branchial arches (c, arrowheads). Localization of Rdd protein was lost in the VEGF MO-injected embryo (d-f ). Scale bars=0.5 mm (A, B). |
