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FIGURE 3
The Xenopus foregut mucociliary epithelium. (a–d) Confocal micrographs of immunostained inner epithelial lining of the Xenopus stage (st.) 45 esophagus, stomach and anterior small intestine (smi). Multiciliated cells are stained for acetylated‐α‐tubulin (Ac.‐α‐tubulin, blue), mucus secretory cells are stained for mucins (PNA, magenta), and cell borders are stained with Phalloidin (actin, green). Magnified areas depicted in (b–d) are indicated by yellow boxes in (a). (e) Mucociliary marker gene expression revealed by in situ hybridization on isolated st. 45 guts. Panc, pancreas; stom, stomach. Image in (a) was reconstructed from multiple individual images |
