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Figure 4. Scleraxis expression in the regenerating froglet blastema was analyzed by section in situ hybridization. A,B:Scleraxis was detected in the lateral region of the early-stage blastema, and expression at this stage was not detected in the medial region. Arrowheads in A indicate signals of scleraxis transcription in the early-stage blastema. C: In situ hybridization on sections of a middle-stage blastema reveals obvious expression in blastema cells (arrowheads). Longitudinal section of a late-stage blastema shows the existence of a few scleraxis-expressing cells (D, arrowhead). Arrows in A–D indicate the signals of scleraxis transcription in cartilage-muscle junctions. Expression of Dermo-1 in the regenerating froglet blastema. Dermo-1 transcripts were detected by in situ hybridization to sections of (E,F) early-stage blastema, (G,G') middle-stage blastema, and (H,H') late-stage blastema. Arrowheads in E–H indicate hybridization signals of Xenopus dermo-1. E: In the subectodermal region, dermo-1 expression was detected from the early stage of the blastema. The boxed region in E is shown in F. G,H: Section in situ hybridization shows that dermo-1 expression continues in the subectodermal region throughout the period of regeneration. G': Higher magnification of G. H': Higher magnification of H. Scale bars in A–E,G,H = 500 μm; in F = 50 μm. Lines indicate the amputation site. |
