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uncxxenopus ectoderm [+] 

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Experiment details for uncx

Yan B et al. (2015) Assay

Microarray identification of novel genes downstream of Six1, a critical factor in cranial placode, somite, and kidney development.

Gene Clone Species Stages Anatomy
uncx.L image:5543082 laevis NF stage 10 to NF stage 20 ectoderm , neural plate , neural tube , olfactory placode

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  Figure 4. Whole-mount in situ hybridization assays at early developmental stages illustrating the common expression patterns of 14 up-regulated genes: Nrp1, Arrb2, BG022063, LOC100037047, BQ399495, Hrpt1, Rbm42-6, LOC414689, Uncx4.1, Xxylt1, Nfya, Tbc1d31, Cdca7l, Papd4-a. For all genes, expression is enhanced in the animal cells (an) of cleavage and blastula embryos (side views). At gastrula stages, expression is throughout the ectoderm, occasionally enhanced in the animal cells (e.g., Arrb2) or the dorsal cells (e.g., Nrp1) (side views). At early neural plate stages, all genes are expressed in the epidermis (epi), often more intensely on the dorsal (d) side, including neural plate (np) and border zone (bz) (all except Nrp1 are side views with anterior [a] to the left; Nrp1 is an anterior view with dorsal to the top). At late neural plate and neural tube stages, expression is strongest in the neural crest (nc), pre-placodal region (ppr), neural tube (nt) and occasionally cement gland (cem) (all are anterior views). For Nrp1, embryos were sectioned in the horizontal plane to demonstrate that the ppr stripe in the whole embryo is due to expression in the deep (d) layer of the ectoderm, not the superficial (s) layer of the ectoderm or the pharyngeal endoderm (phe); ph, lumen of pharynx.

Gene Clone Species Stages Anatomy
uncx.L image:5543082 laevis NF stage 24 to NF stage 33 and 34 brain

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  Figure 5. Whole-mount in situ hybridization assays at tail bud to larval stages illustrating more divergent expression patterns of 14 up-regulated genes: Nrp1, Arrb2, BG022063, LOC100037047, BQ399495, Hrpt1, Rbm42-6, LOC414689, Uncx4.1, Xxylt1, Nfya, Tbc1d31, Cdca7l, Papd4-a. Side views at tail bud (left column) and larval (middle column) stages, with anterior to the left and dorsal to the top, and transverse sections at larval stages (right side) with dorsal to the top. ba, branchial arches; cem, cement gland; ep, epibranchial placodes; epi, epidermis; fb, forebrain; h, heart; hb, hindbrain; IX/Xg, glossopharyngeal/vagal ganglion; l, lens; mb, midbrain; ne, nephric mesoderm; olf, olfactory placode; oto, otocyst; p, pineal; r, retina; spcd, spinal cord; so, somites; Vg, trigeminal ganglion; VIIg, facial ganglion.