|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
tcof1
|
|
tropicalis
|
NF stage 3 (4-cell)
|
animal blastomere
,
animal
,
blastomere
,
animal pole
|
tcof1
|
|
tropicalis
|
NF stage 10
|
dorsal
|
tcof1
|
|
tropicalis
|
NF stage 14
to
NF stage 15
|
anterior neural fold
,
posterior
|
tcof1
|
|
tropicalis
|
NF stage 22
|
branchial crest
,
optic vesicle
,
cranial neural crest
|
tcof1
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
otic vesicle
,
midbrain-hindbrain boundary
,
ventral blood island
,
pharyngeal arch
,
mandibular arch
,
[+]
|
tcof1
|
|
tropicalis
|
NF stage 35 and 36
to
NF stage 40
|
otic vesicle
,
brain
,
midbrain-hindbrain boundary
,
pharyngeal arch
,
mandibular arch
,
[+]
|
utp4
|
|
tropicalis
|
NF stage 3 (4-cell)
|
|
utp4
|
|
tropicalis
|
NF stage 10
|
|
utp4
|
|
tropicalis
|
NF stage 22
|
branchial crest
,
cranial neural crest
,
posterior
|
utp4
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
midbrain-hindbrain boundary
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
|
utp4
|
|
tropicalis
|
NF stage 35 and 36
to
NF stage 40
|
liver
,
head
|
sbds
|
|
tropicalis
|
NF stage 3 (4-cell)
|
|
sbds
|
|
tropicalis
|
NF stage 10
|
|
sbds
|
|
tropicalis
|
NF stage 14
to
NF stage 15
|
cranial neural crest
,
posterior
|
sbds
|
|
tropicalis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
cranial neural crest
,
posterior
|
sbds
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
otic vesicle
,
pharyngeal arch
,
mandibular arch
,
hyoid arch
,
branchial arch
,
[+]
|
sbds
|
|
tropicalis
|
NF stage 35 and 36
to
NF stage 40
|
head
|
rbm28
|
|
tropicalis
|
NF stage 3 (4-cell)
|
|
rbm28
|
|
tropicalis
|
NF stage 10
|
animal hemisphere
|
rbm28
|
|
tropicalis
|
NF stage 14
to
NF stage 15
|
cranial neural crest
|
rbm28
|
|
tropicalis
|
NF stage 22
|
mandibular crest
,
hyoid crest
,
branchial crest
,
cranial neural crest
|
rbm28
|
|
tropicalis
|
NF stage 28
to
NF stage 29 and 30
|
otic vesicle
,
midbrain-hindbrain boundary
,
ventral blood island
,
pharyngeal arch
,
mandibular arch
,
[+]
|
rbm28
|
|
tropicalis
|
NF stage 35 and 36
to
NF stage 40
|
otic vesicle
,
brain
,
midbrain-hindbrain boundary
,
eye
,
pharynx
|
|
|
Fig. 2
Expression of disease-linked ribosome biogenesis factors in X. tropicalis embryos. a Expression of all four genes was detected in the 4-cell and gastrulating embryo. Signal was strongly detected in the neural folds at stage 16 and in the migrating neural crest at stage 22. b At later stages expression of all factors was detected in the facial primordia and developing brain. Note the restricted expression at the mid–hindbrain border and forebrain. Expression was also typically detected in ventral mesoderm and hypaxial muscle precursor cells. utp4 mRNA was strongly detected at the site of the future liver. c Red lines labeled a*, b* and c* in diagrams of stage 10 and 28 embryos represent the planes of section shown in a and b |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
rps14
|
|
tropicalis
|
NF stage 16
|
neural plate
,
anterior neural fold
|
rpl15
|
|
tropicalis
|
NF stage 16
|
|
tcof1
|
|
tropicalis
|
NF stage 18
|
anterior neural fold
|
rps29
|
|
tropicalis
|
NF stage 16
|
anterior neural fold
|
sbds
|
|
tropicalis
|
NF stage 16
|
cranial neural crest
,
posterior
|
rbm28
|
|
tropicalis
|
NF stage 16
|
cranial neural crest
|
rps7
|
|
tropicalis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
anterior neural fold
|
rps19
|
|
tropicalis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
anterior neural fold
,
cranial neural crest
|
rpl26
|
|
tropicalis
|
NF stage 16
|
neural plate
,
cranial neural crest
|
rps17
|
|
tropicalis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
anterior neural fold
,
cranial neural crest
|
rpl35a
|
|
tropicalis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
neural plate border
|
rpl38
|
|
tropicalis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
cranial neural crest
|
|
|
Additional file 2: Comparison of digoxigenin labeled anti-sense and sense mRNA probes for each ribosomopathy gene in stage 16 embryos. Dorsal and anterior views are shown. Note the strong expression detected in the neural plate by the anti-sense mRNA probes while the control sense probed embryos remains unstained. (JPG 922 kb) |