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Fig. 6. Developmental expression of XVax1 analyzed by wholemount
in situ hybridization. (A) Anterior view of an embryo at stage
16. Staining is detected in the anterior neural ridge. (B) Anterior
view of XVax1 expression at stage 19. XVax1 signal is visible in the
ventral forebrain. The optic vesicle (OV) shows no expression.
(C) Ventral/anterior view of an embryo at stage 26. XVax1 transcripts
are detected in the ventral hypothalamus (vh), optic stalk (OS) and
the optic disc (OD). (D) Lateral view at stage 34. XVax1 transcripts
are located the ventral and anterior forebrain. (E) To improve
resolution, a cleared embryo at stage 34 is shown in a dorsal/lateral
view. Strong XVax1 expression is visible in the ventral anterior
forebrain with a sharp dorsal boarder. (F-H) Sections of stained
plastic embedded embryos at stage 34. F; In a sagittal section XVax1
transcripts are detected in the chiasmatic ridge and the ventral
hypothalamus. (G) Parasagittal section showing XVax1 expression in
the eye disc and in the optic stalk. (H) Transverse section at the level
of the optic chiasm. ar, anterior ridge; ba, branchial arch; Ch,
chiasmatic ridge; di, diencephalon; fbv, forebrain vesicle; gu, gut; i,
infundibulum; nc, notochord; OD, optic disk; OS, optic stalk; OV,
optic vesicle; sep, septal/striatal region; vh, ventral hypothalamus;
vtt, ventral tegmental tract. |
