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Fig. 2. Spatial transcription patterns and phenotypic effects observed after ectopic expression of Xvent-1B and Xvent-2B. Transcription patterns were analyzed by whole mount in situ hybridization of Xenopus embryos using Xvent-1B (A) or Xvent-2B (E) antisense RNA. Developmental stages shown for Xvent-1B are: 10.0 (A), 12.5 (B) and 23 (C); Xvent-2B: 10.5 (E), 12.0 (F), 24 (G), 29 (H) and 35 (I). Note that at early stages Xvent-1B expression is restricted more ventrally and lacks anterior expression in the eyes at later stages. Over-expression experiments were carried out by microinjection of either Xvent-1B (D) or Xvent-2B RNA (J) into the dorsal blastomeres of four-cell stage embryos which were then cultured until controls had reached stage 30. Note the complete ventralization and loss of all anterior structures after ectopic expression of Xvent-1B as well as of Xvent-2B. |
