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Figure 1 The spatial distribution of Xvent-2 protein and mRNA in the regenerating tails of Xenopus embryos. (A,B,C,D) Immunostaining; (E,F,G,H) in situ hybridization. A: 0 h; C: 6 h; D,E,F: 20 h after amputation; G,H: amputated tail parts; B: cross-section of the tail in the amputation area. Sections thickness about 0.2 mm. nc, notochord; sc, spinal cord; a, dorsal aorta; v, posterior cardinal vein; av, dorsal lateral anastomosing vessel; f, fin; H, control in situ hybridization with a sense probe. Scale bars: A–E,G,H, 500 µm; F, 100 µm. |
