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Fig. 7. Vimentin expression in the head region of the stage-26 embryo. In a transverse physical section through the head of a stage-26 embryo stained with 14h7 (A), vimentin-expressing head mesenchyme (hm) appears adjacent to the brain and in the region of the mandibular arch (ma). Vimentin-containing processes of cells of the mesencephalon (me) extend in toward the ventricle (nv). Staining in the cement gland is an artifact. In a transverse physical section through an anterior somite of the trunk stained with 14h7 (B), the mesenchyme at the surface of the somites (sm) is expressing vimentin. Vimentin- expressing cells (nv) are present at the margin of the neural tube and have small processes that extend midway to the ventricular surface. There are no vimentin-expressing cells in the ventral region of the neural tube. There appear to be vimentin-expressing mesenchymal cells dorsal to the neural tube (unmarked arrow), c, cement gland; ma, mandibular arch; me, mesencephalon; n, notochord; nt, neural tube; ov, optic vesicle; s, somites. Bars, 50 jxm in both parts. |
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Fig. 8. A & B.
Transverse sections of stage-35 embryos stained for vimentin. In a
transverse section of the head stained with 14h7 (A), vimentin is found in most ofthe head mesenchyme (hm) surrounding the brain, in the meninges (inn), in thecorneal endothelium (ce) and in the mandibular arch (ma). There is also somemesenchyme (em) in the eye. Vimentin is expressed in cells of the mesencephalonand diencephalon (nv). These cells exhibit the characteristic morphology ofmature radial glia, i.e. they are very thin and extend from the margin to the ventricular surface. The staining of the ectoderm is artifactual. A transverse section through an anterior somite of the dorsal trunk (B) reveals that vimentin is expressed in the dorsal fin mesenchyme (dm). Vimentin is also found in the mesenchyme associated with the outer surface of the somites (sm) and in mesoderm below the pronephric ducts (pm). Vimentin-expressing cells are present in the neural tube and extend from the pial to the ventricular surface (nv).....In the area of the embryo ventral to the region illustrated in B (C) are found the lateral body wall and the yolky endoderm ventral to the pronephros. The pronephric rriesoderm is at the top (pm) and below it, between the mesoderm and the endoderm are the vitelline veins (vv) which contain vimentin-expressing mesenchyme. c, cement gland; de, diencephalon; df, dorsal fin; e, eye; me, mesencephalon; n, notochord; nt, neural tube (spinal chord); p, pharynx; pd, pronephric duct; s, somite; vv, vitelline veins. Bars, 50um in A & B, 25um in C. |
