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Figure 2. xWnt6 mRNA is expressed during Xenopus organogenesis. A: Quantitative reverse transcriptase-polymerase chain reaction analysis shows the relative expression levels of Wnt6 mRNA. Wnt6 expression is normalized to histone H4 levels. B-K: xWnt6 mRNA localization determined by whole-mount in situ hybridization using a digoxigenin-labeled xWnt6-specific LNA probe. L-P: Control embryos hybridized with a digoxigenin-labeled nonspecific LNA control probe. Numerical figures indicate the developmental stage. Note, xWnt6 mRNA initially localizes to animal pole and neural fold tissues, then more generally throughout the ectoderm and later in epithelial tissues and in the somites, the developing eye and pronephros. ba, branchial arches; c, cloaca; ea, eye anlage; g, gills; h, heart; hm, hypaxial muscles; nf, neural folds; pa, pronephric anlage; pt, pronephric tubules; s, somites. |
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Figure 3. xWnt6 mRNA is localized within epithelial tissues and developing organs. A-C,E-G,I-K,M-O,Q-S,U-W,Y,Z: Cross-sections of embryos hybridized with a digoxigenin-labeled xWnt6-specific LNA probe. D,H,L,P,T,X,AA: Cross-sections of embryos hybridized with a digoxigenin-labeled nonspecific LNA control probe. Numerical figures indicate developmental stage. ba, branchial arches; ce, corneal epithelium; e, eye; ec, ectoderm; ev, eye vesicle, f, fin; h, heart; hg, hindgut; i, intestine; iev, invaginating eye vesicle; l, lens; lp, lens placode; nf, neural folds; p, pharynx; pt, pronephric tubules; s, somite; vf, ventral fin; vp, velar plate. Scale bars = 200 mu m. |
