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Fig. 4. Spatial pattern of localization of xnff by in situ hybridization and Northern blot analysis. A. The spatial distribution of xnt7 mRNA in stage 17
and stage 26 embryos was analyzed by whole-mount in situ hybridization according to Harland (1991). The stage 17 embryo is observed from above
showing the neural folds which have just closed. The stage 26 embryo is shown from the side (upper panel) and from the dorsal aspect (lower panel).
The arrow points to the anterior of the embryos. Control embryos hybridized with the sense strand xnfl probe did not show any specific hybridization
(third panel under stage 26). The color reaction was carried out for the same time for experimental and control embryos. B. Sections of embryos at
stage 17 and 26 hybridized with the xnff probe. N-notochrod; NT neural tube; S- somites; NP neural plate. C. Localization in dissected embryos.
Ten ug of total RNA isolated from dorsal (D) and ventral (V) regions of stage 25 or stage 45 embryos was electrophoresed, blotted onto Nytran membrane
and hybridized with whole xnf7 cDNA probe. Ethidium bromide staining (bottom) shows equal amounts of RNA loaded into each lane of the
gel. Diagram to the right represents the approximate plane of dissection into dorsal (D) and ventral (V) regions.
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xnf7 (Nuclear factor 7) gene expression in a Xenopus laevis embryo, NF stage 17, as assayed by in situ hybridization. Dorsal view: anterior left |
