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Fig. 7. Expression of Xenopus V2Rs in the olfactory and vomeronasal
epithelial cells of Xenopus larvae. Cross sections of Xenopus
larvae at stage 45 (A) and stage 50 (B–D) were hybridized to
digoxigenin-labeled antisense probes prepared from clones E-1
(A,B,D) and xV2R1 (C). Arrows indicate the receptor-expressing cells.
OE, olfactory epithelium; VNO, vomeronasal organ. Scale bars 50
um. |
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Fig. 3. Analysis of Xenopus V2R expression in the vomeronasal
organs by in situ hybridization. Cross sections of Xenopus vomeronasal
epithelium were stained with hematoxylin (A). The cross
sections were hybridized to digoxigenin-labeled antisense
probes which were derived from clones A-1 (B), B-1 (C), C-1 (D),
E-1 (E), xV2R1 (F), a mixture of A-1, C-1, and E-1 (G), and sense
probes derived from a mixture of A-1, C-1, and E-1 (H). RE, receptor
cell layer; SU, supporting cell layer; VL, lumen of the VNO. Black
spots around the outside of the VNO in A–H (for example, arrow
in F) are melanocyte aggregates. Scale bars 50 m. |
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Fig. 5. Expression of Xenopus
V2Rs in the posterolateral epithelial
area of the principal cavity.
Photographs of hematoxylinstained
transverse sections of Xenopus
laevis upper jaw at intervals
of 400 m (A). Arrow indicates the
V2R-expressing region (A-3). PC,
Principal cavity; MC, middle cavity;
VNO, vomeronasal organ.
Cross-sections of Xenopus nose
were hybridized to digoxigeninlabeled
antisense probes prepared
from clones E-1 (B), xV2R1 (C),
and Go (D). Arrows indicate the
xV2R1-expressing cells (C). Scale
bars 1 mm in A; 100 m in B–D. |
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xv2r1 (pheromone receptor-like) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 66, sagittal sections of the olfactory region. |