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Fig. 2. Expression patterns of Xpreproapelin and Xmsr mRNA. (A) RT-PCR analysis of Xpreproapelin and Xmsr mRNA during development. Ornithine decarboxylase (ODC) was used as an internal control. (B, C) Expression of Xpreproapelin mRNA at stage 10. Xpreproapelin mRNA was detected in the marginal zone, at the bilateral area of the dorsal lip (arrowheads). Inset in panel B shows a magnified view of the expression. (D, E) Expression of Xpreproapelin mRNA at stage 18, in the anterior ventral region of the embryo (arrowheads). (F, G) Expression of Xmsr mRNA at stage 10, in the marginal zone except for dorsal marginal zone (arrowheads). (H, I) Expression of Xmsr mRNA at stage 18, in the anterior ventral region of the embryo (arrowheads). (J–L) Expression of Xpreproapelin mRNA at stage 22, in regions lateral and posterior to the cement gland (CG), the dorsal posterior part of the embryo, and the dorsal midline. (M–O) Expression of Xmsr mRNA at stage 22, in areas lateral and posterior to the cement gland (CG), the dorsal posterior part of the embryo, and the dorsal midline. (P–R) Expression of Xpreproapelin mRNA at stage 30, around the anterior and posterior cardinal vein (ACV white arrowhead, PCV black arrowhead), aortic arcs (AA, arrowhead), dorsal longitudinal anastomosing vessel (DLAV, arrowhead), notochord (NC), neural tube (NT), and tailbud. (S–U) Expression of Xmsr mRNA at stage 30, in the anterior and posterior cardinal vein (ACV white arrowhead, PCV black arrowheads), aortic arcs (AA, arrowheads), endocardium (EC arrowhead), vascular vitelline network (VVN arrowhead), and tailbud. B, F, L, O: dorsal view;, C, G: vegetal view, dorsal upward, D H; anterior view, E, I, K, N, R, U: ventral view, J, M, P, Q, S, T: lateral view. Panels Q and T are magnified views of the dorsal part of the cleared embryos shown in panels P and S, respectively. |
