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Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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atp1a1 | xenopus | sclerotome [+] |
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Experiment details for atp1a1
Davies CS et al. (1996) AssayPrimary sequence and developmental expression pattern of mRNAs and protein for an alpha1 subunit of the sodium pump cloned from the neural plate of Xenopus laevis.
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FIG. 5. The spatial distribution of pXen9 RNAs during gastrulation and neurulation. (A) Longitudinal section through the dorsal lip of an early gastrula (stage 10.5). Note absence of signal in outer layer of dorsal ectoderm behind the dorsal lip, with a sharp boundary between nonexpressing and expressing cells (arrow). The nonexpressing cells are destined to form the outer layer of the neural plate. DL, dorsal lip. (B) Embryo at stage 11. The region of nonexpressing dorsal ectoderm cells now extends further back (arrow), paralleling invagination of the mesoderm. (C, D) Longitudinal sections through dorsal (C) and ventral (D) regions of an embryo at the end of gastrulation (stage 12). Note the lack of expression in the outer layer of the dorsal ectoderm, the putative neural plate. DL, dorsal lip; VL, ventral lip; YP, yolk plug. (E) Stage 14, transverse section through the neural plate. Note transcript expression is lower in superficial cells than deep cells of the neural plate (NP). The notochord (no), somites (som), and endoderm cells lining the archenteron show transcript expression. (F) Stage 17. Expression is most intense in the neural plate (NP) and lateral ectoderm (LE). The notochord (no) and somites (som) now show more intense expression than endoderm cells lining the expanding archenteron. (G) Stage 22. NT, neural tube; no, notochord; som, somites. (H) Stage 36. All dorsal structures now show intense expression of Na pump a1 subunit mRNAs. (I) Sense control, stage 17. (J) Stage 36; expression of collagen mRNAs. Note expression restricted to the notochord and floor plate (FP). (K) Stage 36. Expression of actin mRNAs is restricted to the somites. Bars, 100 mm for all except H, J where bars are 25 mm. |