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Fig. 5. Gastrula and neurula stage expression. In situ hybridization to gastrula and neurual stage Xenopus embryos with Endocut [darmin], Vito [slc5a8.1] , β3 [] and Fetuinish (fetub) antisense probes. The two left panels show whole mount in situ preparations to stage 11 gastrula embryos, from a vegetal view (extreme left) and a mid-sagital section, with dorsal/anterior to the left. The superficial endoderm of the blastopore lip (red arrow head) expresses Vito and β3 but not Endocut. The stage 15–18 embryos are bisected mid-sagitally and shown with anterior to the left and dorsal up. The stage 20 embryos are shown in cross section with dorsal up. |
