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Figure 3. Spatiotemporal expression of XFGFRL1/Xndk at late gastrula to neural tube stages visualized by whole-mount in situ hybridization. Hybridization probes used for XFGFRL1 and XFGF8 are as indicated in the pictures (XFGFRL1 in black; XFGF8 in blue). ldquo cleared, rdquo embryos were treated with benzyl benzoate/benzyl alcohol to become transparent. Numbers indicate developmental stages (Nieuwkoop and Faber, [1967]). A: Lateral view of late gastrula. Anterior toward the left; dorsal is upward. B: Lateral view of early neurula. Anterior toward the left; dorsal is upward. C: Sagittal section of early neurula (stage 13/14). D: Transverse section of early neurula (stage 13/14). The section corresponds to the position shown by a line with d in B. E: Anterolateral (left) and lateral view (middle; anterior toward the left; dorsal is upward) of XFGFRL1 and XFGF8 (right) expression at neurula. Arrowhead, ventrolateral expression in a crescent shape. F,G: Horizontal (F) or transverse (G) section of neurula (stage 17/18). The planes of section are indicated by a line with f or g in E. Arrowheads in lower panels indicate that crescent-shaped expression (see E, right panel) is seen in the sensorial layer of epidermis. H: Sagittal section of neurula (stage 17/18). I: Lateral (left and middle panels; anterior toward the left; dorsal is upward) and anterior view (right panels; dorsal is upward) of neural tube stage embryos. Crescent-shaped expression (see E, right panel) is separated into two stripes as indicated by arrowheads (see I, lower panels). C,D,F-H: Sections of stained embryos are shown in brightfield (left) and DAPI (4 prime ,6-diamidine-2-phenylidole) staining (right). XFGFRL1, Xenopus fibroblast growth factor receptor-like 1; XFGF8, Xenopus fibroblast growth factor-8; ae, anterior endoderm; anr, anterior neural ridge; ar, archenteron roof; bc, blastocoel; ey, eye; fb, forebrain; lp, lateral plate; mhb, midbrain-hindbrain boundary; no, notochord; op, otic placode; pcp, prechordal plate; pm, paraxial mesoderm; sm, somitic mesoderm. Scale bars = 200 mu m in C,D,F-H. |
