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Figure 1. Expression of FRG1 transcript and protein during development. A: Analysis of Xenopus laevis frg1 transcript levels across developmental stages using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). B-G: In situ hybridization using the Xenopus laevis frg1 transcript at stage (St.) 26 (B), St. 32 (C), and St. 38 (E,F). Sense frg1 controls are shown for St. 32 (D) and St. 38 (G). Arrowheads point to migrating hypaxial muscle. H-J: FRG1 immunostaining on St. 18 (H), St. 24 (I), and St. 38 (J) embryos showing FRG1 antibody (upper) and secondary antibody alone control (lower). K,L: Stage 30 FRG1 immunostained embryos were sectioned sagitally (K) or transversely (L). Arrows point to aligned nuclei in the somite stained with FRG1 antibody. np, neural plate; ps, pronephric sinus; nt, neural tube; nc, notochord; is, intersomitic region. |
