| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| gbx2.2 | xenopus | foregut [+] |
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Experiment details for gbx2.2
von Bubnoff A et al. (1996) AssayThe Xenopus laevis homeobox gene Xgbx-2 is an early marker of anteroposterior patterning in the ectoderm.
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| Fig. 6. Localization of Xgbx-2 expression in the hindbrain. Embryos were hybridized alone or in combinations with Xgbx-2, Krox-20, or En-2. For all embryos, a lateral view is shown with dorsal at the top and anterior to the left. The magnification is the same in all photographs. All embryos are cleared. Arrowhead in A, B, D-F: anterior border of Xgbx-2 expression. (A-C) Stage 21. (A) Embryo hybridized with Xgbx-2 alone. Xgbx-2 is also expressed in the head endoderm (arrow). (B) Embryo cohybridized with Xgbx-2 and Krox-20. Note the gap between the Xgbx-2 stripe and the rhombomere 3-stripe (3) of Krox-20, which is a length of approximately one rhombomere. Krox-20 is also expressed in neural crest (nc) migrating into the third visceral arch (Bradley et al., 1992). (C) Embryo hybridized with Krox-20 alone. (DG) Stage 31. (D) Embryo cohybridized with Xgbx-2 and Krox-20. The anterior Xgbx-2 expression border (arrowhead) lies at least 2 rhombomere lengths anterior to the rhombomere 2/3 boundary. (E) Embryo hybridized with Xgbx-2 alone. (F) Embryo cohybridized with En-2 and Xgbx-2. The En-2 stripe does not appear to be widened compared to (G). The anterior border of Xgbx-2 expression (arrowhead) would be predicted to lie approximately halfway into the En-2 expressing region (compare to E). (G) Embryo hybridized with En-2 alone. En-2 is also expressed in the mandibular arch (arrow). | ||||||||||||||||
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| Fig. 3. Spatial expression of Xgbx-2 during early development. The expression was analyzed by whole mount in situ hybridization of albino embryos. In all pictures, anterior is to the left. (A) Dorsal view of a stage 11 embryo. Note the wide gap at the dorsal midline and the distance of the expression from the blastopore lip (b). (B) Dorsolateral view of a stage 11 embryo. (C) Dorsal view of a stage 13-14 embryo. The gap in expression at the dorsal midline has narrowed. Note the sharp anterior expression border in the neuroectoderm (n). (D) Lateral view of a stage 13-14 embryo. Xgbx-2 is expressed in the neuroectoderm (n) and in epidermal ectoderm (e). (E) Dorsal view of a stage 15 embryo. The neurectodermal expression is concentrated in a pair of anterior stripes (n). The epidermal expression has a sharp anterior border (e). There is additional expression in the presumptive posterior spinal cord (s). (F) Dorsal view of a stage 19-20 embryo. Labeling as in (E). (G) Lateral view of a stage 19-20 embryo. The lateral epidermal expression (e) shows a sharp anterior border and gradually tapers off towards the posterior end. Labeling as in (E). (H) Dorsal view of a cleared stage 22-23 embryo. Xgbx-2 is expressed in the anterior neuroectoderm (n), in the otic vesicle (o), in a pair of lateral stripes anterior to the otic vesicle, in the neural tube (nt) posterior to the otic vesicle, in the posterior spinal cord (s), and in the future branchial arches (lateral to the otic vesicle). (I) Dorsal view of an uncleared stage 22-23 embryo. Expression is in the region of the neural crest (arrowhead) ventrally and posterioventrahy to the otic vesicle. (J) Dorsal view of a cleared stage 31 embryo. Note the crescent-shaped expression in the dorso-medial part of the otic vesicle (0). Labeling as in (H). (K) Lateral view of a cleared stage 31 embryo. Note expression in the dorsal and ventral part of the anterior hindbrain (n). Them is weak expression in the region of the visceral arches (va). Labeling as in (H). (L) Dorsolateral view of a cleared stage 31-32 embryo, showing Xgbx-2 expression in the neural tube dorsal to the eye (arrowhead). |