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golm1xenopus blastocoel 

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Experiment details for golm1

Xenopus as a model system for the study of GOLPH2/GP73 function: Xenopus GOLPH2 is required for pronephros development.

Xenopus as a model system for the study of GOLPH2/GP73 function: Xenopus GOLPH2 is required for pronephros development.

Gene Clone Species Stages Anatomy
golm1.L laevis NF stage 10 blastocoel
golm1.L laevis NF stage 13 blastocoel

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  Figure 4. The expression pattern of golph2 during early development. A. RT-PCR analysis of the temporal expression pattern of golph2. UE, unfertilized egg. RT(-), negative control of reverse transcription. Ornithine decarboxylase (ODC) was used as the RNA loading control. B. Western blotting analysis of golph2 expression during Xenopus embryogenesis. Beta-actin served as a protein loading control. C. Whole-mount in situ hybridization was conducted using a digoxigenin-labeled antisense probe for golph2. C. Embryos bisected prior to in situ hybridization. Orientation: animal top, dorsal right (C, D); dorsal top, ventral bottom (E, F), stages as indicated. The expression of golph2 is detected in the dorsal marginal zone and the derivative mesoderm at the gastrula stage. G. Lateral view, anterior towards the left; golph2 expression in the pronephros is detected between stages 25 and 39. Expression of golph2 in the otic vesicle and cement gland is also shown (H). Arrow heads indicate the signal.