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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
vax1
|
|
laevis
|
NF stage 19
to
NF stage 23
|
eye primordium
|
vax2
|
|
laevis
|
NF stage 19
to
NF stage 23
|
eye primordium
|
gnpat
|
|
laevis
|
NF stage 17
|
pre-chordal neural plate
,
dorsal
,
anterior neural ridge
|
gnpat
|
|
laevis
|
NF stage 19
|
eye primordium
,
neural tube
,
anterior neural ridge
|
gnpat
|
|
laevis
|
NF stage 20
to
NF stage 22
|
forebrain
,
hindbrain
,
eye primordium
,
neural tube
|
gnpat
|
|
laevis
|
NF stage 23
|
forebrain
,
hindbrain
,
optic vesicle
,
neural tube
|
gpat3
|
|
laevis
|
NF stage 17
|
eye primordium
,
dorsal
,
anterior neural ridge
|
gpat3
|
|
laevis
|
NF stage 19
|
cement gland primordium
,
eye primordium
,
neural tube
|
gpat3
|
|
laevis
|
NF stage 20
to
NF stage 22
|
forebrain
,
hindbrain
,
eye primordium
,
neural tube
|
gpat3
|
|
laevis
|
NF stage 23
|
forebrain
,
hindbrain
,
optic vesicle
,
neural tube
|
gpat4
|
|
laevis
|
NF stage 12
to
NF stage 13
|
involuting marginal zone
|
gpat4
|
|
laevis
|
NF stage 19
|
eye primordium
,
neural tube
|
gpat4
|
|
laevis
|
NF stage 20
to
NF stage 22
|
forebrain
,
hindbrain
,
cement gland primordium
,
eye primordium
,
neural tube
|
gpat4
|
|
laevis
|
NF stage 23
|
forebrain
,
hindbrain
,
cement gland primordium
,
optic field
|
tbx3
|
|
laevis
|
NF stage 17
|
cement gland primordium
,
optic field
|
tbx3
|
|
laevis
|
NF stage 19
|
cement gland primordium
,
eye
|
tbx3
|
|
laevis
|
NF stage 20
to
NF stage 23
|
olfactory placode
,
cement gland primordium
,
eye
,
adenohypophyseal placode
|
gpam
|
|
laevis
|
NF stage 12
to
NF stage 13
|
involuting marginal zone
,
involuted dorsal mesoderm
|
gpam
|
|
laevis
|
NF stage 17
|
pre-chordal neural plate
,
dorsal
|
gpam
|
|
laevis
|
NF stage 19
|
eye primordium
,
neural fold
|
|
|
Fig. 4. mRNA expression of GPATs and DHAPAT during neurulation and early organogenesis detected by whole-mount in situ hybridization. Expression is first detected at late gastrulation (stage 12/13) and early neurulation. All the GPATs and DHAPAT are expressed in a region around the blastopore (b) and along the anterior-posterior embryonic axis, towards the dorsal midline (dm). At stage 17, GPATs and DHAPAT are expressed in the nervous system, including the neural tube (nt), hindbrain (hb), the forebrain (fb) and the eye primordia (ep). This pattern becomes more evident at stage 19. Nervous system expression is observed in dorsal and anterior views of embryos at stage 20 and older. Vax2 and ET are two genes encoding transcription factors involved in early eye development, and were used for reference. Stage 20/22 left panel, dorsal view; Stage 17, 19, 20/22 right panel and 23, anterior view. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gpat2
|
|
tropicalis
|
NF stage 40
|
otic vesicle
,
brain
,
spinal cord
,
head region
,
germ cell
,
[+]
|
gpam
|
|
laevis
|
NF stage 26
|
brain
,
spinal cord
,
head region
,
eye
,
pronephric mesenchyme
|
gpam
|
|
laevis
|
NF stage 32
|
otic vesicle
,
brain
,
spinal cord
,
head region
,
pronephric duct
,
[+]
|
gpam
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
spinal cord
,
pharyngeal arch
,
eye
,
[+]
|
gpam
|
|
laevis
|
NF stage 37 and 38
|
otic vesicle
,
spinal cord
,
head region
,
pharyngeal arch
,
eye
,
[+]
|
|
|
Fig. 6. mRNA expression of GPAT1 and GPAT2 in different organs detected by whole- mount in situ hybridization. (A) GPAT1 is expressed during kidney and liver organogenesis. Whole-mount in situ hybridization with the GPAT1 antisense riboprobe of embryos at the indicated stages is shown in the left column. Higher magnifications of the kidney and liver regions are shown in a lateral view in the right two panels (anterior to left). Eye (E), kidney (K) (early expression detected at stage 26) and liver (L) (early expression detected at stage 33/34) are shown. (B) GPAT1 and GPAT2 show a different expression pattern at stage 40. GPAT1 and GPAT2 are both expressed in the nervous system (E, eye; B, brain; and SC, spinal cord). Further, GPAT1 is strongly expressed in liver (L) and kidney (K), whereas GPAT2 is expressed in presumptive gonad (testis-T) and weakly in kidney. |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 28, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gnpat
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
forebrain
,
head region
,
eye
,
pronephric kidney
,
[+]
|
gnpat
|
|
laevis
|
NF stage 37 and 38
|
otic vesicle
,
head region
,
pronephric duct
,
eye
,
pronephric kidney
|
gpat2
|
|
tropicalis
|
NF stage 33 and 34
|
otic vesicle
,
brain
,
spinal cord
,
head region
,
somite
,
[+]
|
gpat2
|
|
tropicalis
|
NF stage 37 and 38
|
brain
,
spinal cord
,
head region
,
somite
,
pronephric duct
,
[+]
|
gpat3
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
forebrain
,
hindbrain
,
spinal cord
,
head region
,
[+]
|
gpat3
|
|
laevis
|
NF stage 37 and 38
|
forebrain
,
hindbrain
,
head region
,
pronephric duct
,
liver
,
[+]
|
gpat4
|
|
laevis
|
NF stage 33 and 34
|
otic vesicle
,
forebrain
,
hindbrain
,
spinal cord
,
pronephric duct
,
[+]
|
gpat4
|
|
laevis
|
NF stage 37 and 38
|
otic vesicle
,
spinal cord
,
head region
,
pronephric duct
,
liver
,
[+]
|
gpam
|
|
laevis
|
NF stage 37 and 38
|
otic vesicle
,
head region
,
pronephric duct
,
liver
,
eye
,
[+]
|
|
|
Fig. 5. mRNA expression of GPATs and DHAPAT in embryos and in kidney between stages 32 and 37/38 as detected by whole-mount in situ hybridization. mRNA for all GPATs and DHAPAT are present in the kidney (K), as well as in the nervous system, including the eye (E), otic vesicle (OV), spinal cord (SC) and branchial arches (BA). GPAT1 and GPAT4 are also expressed in the liver (L). Lateral views at the indicated stages (anterior to left). GPAT1, GPAT2, GPAT4, and DHAPAT are expressed in the pronephric tubules (arrows) and ducts (arrowheads), but GPAT3 is not expressed in the pronephric ducts (white arrowhead). |
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gpam ( glycerol-3-phosphate acyltransferase, mitochondrial ) gene expression in Xenopus laevis embryos, NF stage 40, as assayed by in situ hybridization, lateral view, anterior left, dorsal up. |