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Figure 3. Developmental expression of Gsh2 [now called gsx2] . A-O, Q-T, V-A2: Gsh2 WISH. P, U: Double WISH as indicated. Q, V, X: Cleared embryos. I-L: Transverse vibratome sections (40-50 mu M). R-U, W, Y-A2: Transverse microtome sections (14 mu m). A: Dorsal, late gastrula stage 13. B: Dorsal, mid-neurula stage 17. C: Anterior, stage 17. D, E: Stage 21. D: Lateral. E: Bisected through hindbrain. F-H: Early tailbud stage 25. F: Dorsal. G: Anterior. H: Lateral. I-L: Sections through: (I) forebrain, early tailbud stage 24, (J) eye, tailbud stage 31, (K) anterior trunk, stage 24, (L) posterior trunk, tailbud stage 27. M-O: Stage 30. M: Lateral. N: Anterior. O: Dorsal. P: Head, lateral stage 31. Q: Head, lateral, tailbud stage 29. R-T: Serial sections through forebrain. U: Section through posterior hindbrain, stage 31. V: Head, dorsal, late tailbud stage 40. W: Frontal section through head, stage 40. X-A2: Larval stage 47. X: Head, dorsal. Y-A2: Sections through: (Y) LGE, (Z) prethalamus, (A2) ventral pharynx.
Key: en, lateral endoderm; hb, hindbrain; lge, lateral ganglionic eminence; mge, medial ganglionic eminence; nc, notochord; ob, olfactory bulb; pth, prethalamus; r8, rhombomere 8; r7-8, rhombomeres 7-8; rpe, retinal pigment epithelium; sc, spinal cord; vp, velar plate; vz, ventricular zone; zli, zona limitans intrathalamica. |