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Fig. 1. Whole-mount in situ analysis of XHRT1, esr9, esr10, Xhairy1 and Xhairy2b expression in comparison with X-Delta-1 and xWT1 in the pronephros region (arrowheads) of early tailbud stage embryos. Nieuwkoop-Faber stages are indicated (Nieuwkoop and Faber, 1967). (A-D) Comparison of the expression of XHRT1, X-Delta-1 and xWT1 in stage 20 embryos. (A) Whole embryo, lateral view, anterior right. (B-D) Transversal sections. XHRT1 expression coincides with that of xWT1 around the pronephros anlagen and surrounds that of X-Delta-1. (E-N) Comparison of the expression of XHRT1, esr9, esr10, Xhairy1 and Xhairy2b in stage 22-23 embryos. (E,G,I,K,M) Whole embryos, lateral views, anterior right. (F,H,J,L,N) Transversal sections. Note that esr9 and esr10 are co-expressed with XHRT1 around the dorsal border of the pronephros anlagen, and that Xhairy1 and Xhairy2b expression is detected in both mesodermal layers and in the ectoderm. e, ectoderm; pn, pronephros; l, lateral intermediate mesodermal layer; m, medial intermediate mesodermal layer. |
