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FIG. 8. (A, B) In situ hybridization of stage 63 cross sections. (A) Exterior section showing expression of gene 5 exclusively in subepidermal
fibroblasts. (B) Interior section demonstrating the absence of gene 5 expression. Sketch of stage 62 tadpole (Nieuwkoop and Faber, 1956)
showing the locations (C–E) in the tadpole where the three planar images were photographed. (C–E) In situ hybridization with a mixture
of antisense probes of both adult keratin and gene 5 in stage 62 planar sections across the body and tail, arrows point out adult keratin (AK)
and gene 5 expression. Sections in C–E are oriented anterior to posterior from left to right. Scale bars, 100 mm. |
