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id4xenopus hindbrain 

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Experiment details for id4

Liu KJ and Harland RM (2003) Assay

Cloning and characterization of Xenopus Id4 reveals differing roles for Id genes.

Gene Clone Species Stages Anatomy
id4.S laevis NF stage 33 and 34 hindbrain
id4.S laevis NF stage 37 and 38 hindbrain

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  Fig. 3. Id4 mRNA expression pattern in the early embryo. (A, C, E, G) Dorso-anterior views at stage 13, 15, 17, and 19. (B) Lateral view stage 13. (D, F) Dorsal view stage 15 and 17. (H) Lateral view stage 19. (I, K) Lateral and dorsal views, stage 24. (J, L) Lateral view of head staining, stages 32 and 34. (M) Lateral view stage 37. Embryos show staining in the anterior region of the embryo corresponding to the cement gland (yellow arrowhead). By stage 19, expression is also obvious bilaterally in the olfactory placode (black arrow), eye primordia (blue arrowhead), and in the trigeminal ganglia (red arrow). Dorsal and lateral views show expression in the Rohon–Beard neurons (black arrowhead). In later stages, Id4 is expressed in the liver (marked with a yellow L), branchial arches (black b), kidney (red arrowhead marks tubules in J, L, and M, red arrow marks duct in M), and in migrating melanophores (small red arrowheads in M).

Gene Clone Species Stages Anatomy
id4.S laevis NF stage 37 and 38 hindbrain

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  Fig. 4. mRNA expression of X. laevis Id2 (A–D), Id3 (E–H), and Id4 (I–L). Yellow lines mark plane of section. (A, E, I) Both Id2 and Id3 are expressed in the nasal pit (blue arrowhead) and in the otic vesicle (left of asterisk). Migrating cells (small red arrowheads) mark ventrally migrating muscle cells in (A) (Id2), the leading edge of migrating muscle cells in (E) (Id3), and migrating melanophores in (I) (Id4). (D, H, L) Id2 is expressed in both the dorsal and ventral portions of the myotome, in a region corresponding to the nuclei of the muscle cells (D–M). Id3 is expressed in the most dorsal and ventral portions of the myotome (H–M). Id4 is expressed weakly in a similar pattern to Id2. Both Id2 and Id3 are expressed in punctate cells in the fin mesenchyme. Id4 is not expressed in the fin mesenchyme. (The chordoneural hinge is marked with a red arrowhead, the inner fin with an open yellow arrowhead and the outer fin with a closed yellow arrowhead.) Id4 is also expressed in the lateral line (io: infraorbital, a: aortic) marked in (I).