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Fig. 4. Vg1 RBP mRNA distribution in embryos. Whole mount in situ hybridization reveals the distribution of Vg1 RBP mRNA in embryos from stages 3 (4-cell stage, (A)); 20, (B); 26, (C); 30/31, (D,E). In (E), the embryo, following staining, was re®xed in Bouin's. In (B) the arrowheads indicate the area of putative neural crest delaminating and migrating from the midbrain (mb) and hindbrain. The cement gland (cg) is unlabeled. Ot, optic tectum; e, eye; ov, otic vesicle; ba, branchial arches; pn, pronephros; bi, blood islands. |
