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Fig. 3. Comparison of xIsm and xFgf-8 expression. (A,D) Stage 10 embryos in vegetal view; note that xIsm and xFgf-8 expression overlap in the ventrolateral blastopore lip (vbl). (B,E) Stage 17 embryos in dorso-vegetal view showing expression of xIsm and xFgf-8 in the anterior notochord (n). (C,F) Stage 20 embryos in anterior view; note overlapping expression of xIsm and xFgf-8 in cranial neural crest (nc), anterior edge of neural plate (anp), and midbrain–hindbrain border (arrowheads). (G–I) Stage 30 embryos in lateral view. (G) Whole-mount in situ hybridization depicting xIsm expression in the branchial arches (ba), diencephalon (di), ear placode (e), and isthmus (arrowhead). (H) xFgf-8 expression at the same stage. (I) Double in situ hybridization of xIsm and xFgf-8; note the overlap of expression in branchial arches, diencephalon and isthmus (arrowhead), as well as the complementary expression in the ear placode. |