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NIHFig.
5. Localization of integrin a6 subunit mRNA in stage 32 tailbud Xenopus embryos by whole-mount in situ hybridization. Views of stage
32 Xenopus embryos probed with either antisense (A,C-K) or sense (B) digoxigenin-labeled transcripts. The rostral end of each embryo is to
the right in A-H). (C) Integrin a6 mRNA is expressed in discreet regions throughout the developing nervous system including the Schwann
cells of the seventh, eighth and tenth (VII, VIII and X) cranial nerves (arrows), the olfactory placode (op), the otic vesicle (ov), (D) the
trigeminal ganglia (tg) and lens (l), and (F) inter-neurons in the spinal cord (in). In the developing brain (H), a6 mRNA is expressed in the cells
of the ventricular layer (v). In the developing kidney (E), integrin a6 mRNA is expressed in the pronephros (pn) and (G) the elongating
pronephric duct (pnd). Transverse sections of whole-mount stained embryos are presented at the levels of the (I) hindbrain, (J) rostral spinal
cord and (K) mid-thoracic region. a6 mRNA in the neural tube is detected in two rows of laterally symmetrical interneurons whose cell bodies
reside midway along the dorsal-ventral axis of the neural tube (arrowheads). (I) In rostral regions of the embryo, a6 mRNA is present in the
otic vesicle (ov) and within the pronephros (pn), and (J) more caudally in the pronephric duct (pnd). |
