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FIG. 7. Spatial patterns of integrin {J1 mRNA expression analyzed by whole mount i-n situ hybridization. Whole mount in situ hybrid izations
demonstrate that #1 n1RNA is expressed in a wide variety of embryonic tissues. (A) Vegetal view of a stage 11 gastrula; note increased levels of
expression along the blastopore (arrow). (B) Dorsal view of a stage 20 neurula, staining in the notochord, somites. and brain. (C) Side view of the
same embryo, staining in dorsal mesodermal and ect~ermal derivatives. (D) Side view of a stage 25 neurula with reduced levels of expre~:~sion in
the notochord. Arrows show areas of pronounced expression in the brain. (E) Detail of the head of the same embryo in (D); arrows indicate
staining in the branchial arches. (F) Stage 28 neurula with high levels of expression in the posterior notochord (arrows). (G) Stage 33/34 tailbud
embryo with decreased levels of expression in the notochord and myotomes but continued staining at the tipo( the tail (arrow). (H) Stage 37/38
tail bud embryo with abundant expression of {J1 mRNA in the region of the heart (arrow) and decreased signal in dorsal structures. |
