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Fig. 2. Kidins220 localization pattern in somites and spinal neurons of DAB-stained embryos. Lateral views, anterior to the left, dorsal to the top (A-D). Sagittal section of a Kidins220-immunostained embryo (A) and Hoechst-counterstaining (B). The red square brackets indicate paraxial mesoderm. Whole-mount ab34790 anti-Kidins220-immunostaining (C) and Ab12101 anti muscle-immunostaining (D). Coronal sections of Kidins220 (Hoechst-counterstained) and Ab12101-stained embryos, respectively (E,F). The arrows in (E) show the Kidins220 signal in lateral regions of spinal cord. High magnification of a Kidins220-positive intersomitic junction (G). Transverse sections of Kidins220 and acetylated tubulin-immunostained embryos, respectively (H,I): the arrowheads show the exit point of a motor neuron that in (H) resulted broken because of sectioning. Sagittal section of a stage 42 embryo (J) and Hoechst-counterstaining (K). The arrowheads in (J) indicate motor neurons. The square brackets in (E,J,K) span somite length. Acet. tub.= acetylated tubulin; nt= neural tube; ij= intersomitic junction. Scale bars: (A,B,H,I) 50 um; (C,D) 250 um; (E,F) 25 um; (G,J,K) 10 um. |
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Fig. 5. Sagittal cryosections of stage 42 embryos double-labeled for Kidins220 (red) and acetylated tubulin (green) by immunofluorescent staining. Kidins220-positive signal was present in dorsal and ventral regions of the spinal cord as well as in motor neurons (arrows) (A) as shown by the acetylated tubulin staining (B,C). Magnification of motor neurons double-stained by Kidins220 (D), acetylated tubulin (E) and merged (F). The dashed bar in (D) spans the somite length. Kidins220 protein in eye of st. 33-34 DAB-stained embryos (G) and st. 42 embryos double-immunolabeled for Kidins220 and acetylated tubulin (H-J). The open arrowhead in (H,J) indicates the Kidins220 signal in the optic nerve. The dashed circle indicates the position of lens in the eye. The white arrow in (H,J) denotes the presence of Kidins220 signal in a facial muscle near the eye. The white arrowheads in (H,J) indicate Kidins220 and acetylated tubulin-positive nerve fibers present in epidermis. ep, epidermis; l, lens; rgc, retinal ganglion cell; s, somite; sc, spinal cord; *, ciliated cell. Scale bars: (A-C) 50 um; (D-F,G-J) 25 um. |
