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l1camxenopus notochord [+] 

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Experiment details for l1cam

Visualizing flow in an intact CSF network using optical coherence tomography: implications for human congenital hydrocephalus.

Visualizing flow in an intact CSF network using optical coherence tomography: implications for human congenital hydrocephalus.

Gene Clone Species Stages Anatomy
l1cam tropicalis NF stage 28 notochord

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  Supplemental Figure 4. Validation of l1cam F0 CRISPR mutant. (a) DNA gel showing the PCR product for each l1cam F0 CRISPR mutant and control, before and after cutting with T7 endonuclease I. Mid sagittal OCT image of control and two independent l1cam F0 CRISPR mutants, the later showing stenosis of the cerebral aqueduct (red arrowheads). (b) At stage 28 l1cam mRNA expression can be seen in the eye, brain, spinal cord and the notochord using in-situ hybridization. At stage 45 it is weakly expressed in forebrain but a strong expression is seen in the mid and hind brain region. (c) Immuno-fluorescence against l1cam protein shows loss of the signal in mutant as compared to the control stage 45 brain.

Gene Clone Species Stages Anatomy
l1cam tropicalis NF stage 28 notochord

  l1cam (L1 cell adhesion molecule) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.