| |
Fig. 6. Over-expression of lmx1b and its potential binding partners affects nephrin expression. mRNAs of lmx1b and its potential binding partners were injected either alone (panel A) or in combination (panel B), together with lacZ, into one cell of 2-cell embryos. Red Gal staining followed by nephrin in situ hybridisation was carried out at stage 33/34 in order to assess any glomus phenotype. For each embryo, the injected side (indicated by an asterisk, panels a–d) was compared to the uninjected side (panels e–h). Injection of lmx1b mRNA alone did not induce any significant change in nephrin staining (A, compare panel a to e), whereas injection of lim1 or ldb1 alone resulted in the formation of an enlarged and reduced nephrin domain respectively (A, b and f; A, c and g). These phenotypes can be partially rescued by the co-injection of lmx1b (B, b and f; B, c and g). Co-injection of lim1 and ldb1 rescued both phenotypes, the embryos displayed on the injected side a nephrin domain, similar in area to the uninjected side (B, a and e). Injection of E47 alone or in combination with lmx1b did not induce any statistically significant phenotype (A, d and h and B, d and h). |
