|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nrp1
|
|
laevis
|
NF stage 22
|
forebrain
,
telencephalon
,
diencephalon
,
midbrain
,
hindbrain
,
[+]
|
otx2
|
|
laevis
|
NF stage 22
|
forebrain
,
cement gland primordium
,
eye
,
optic vesicle
,
anterior
|
egr2
|
|
laevis
|
NF stage 24
|
hindbrain
,
cranial neural crest
,
rhombomere R3
,
rhombomere R5
,
anterior branchial crest
|
gbx2.1
|
|
laevis
|
NF stage 22
|
hindbrain
,
midbrain-hindbrain boundary
,
otic placode
,
cranial neural crest
,
rhombomere
|
myod1
|
|
laevis
|
NF stage 22
|
somite
,
muscle
|
|
|
Fig. 5. Triple PG1 knockdown severely affects hindbrain patterning. Morpholinos for each of the Hox PG1 genes (A1/B1/D1MO) were co-injected into the lhs of the embryo (B,D,F,H,J). Non-injected controls are also shown (A,C,E,G,I). The hindbrain region is shown, with anterior to the top. Expression of the neural marker Nrp1 (A,B: 100%, n=11), the anterior marker Otx2 (C,D: 100%, n=10), Gbx2, which is expressed in r1 (E,F: 77%, n=13) and Krox20 (G,H: no stripes 41%; one faint stripe 35%, n=71), was analysed. Dotted line indicates the midline. In later embryos (st. 46), the neural antibody 2-G9 was used to show the morphology of the hindbrain [NIC, I; A1/B1/D1MO, injected on the left-hand side (lhs) *, J: 100%, n=5]. Rhombomere boundaries are marked. |