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Fig. 2. Expression patterns of genes responsible for segmental patterning are altered in embryos lacking Mespo function. For all embryos, anterior is to the left. (A–K) One VMZ cell of four-cell-stage embryos injected with control MO (A, D, F, H and J), or Mespo MO (B, E, G, I and K) or Mespo MO plus 35 pg pCS–mMespo (C) and the embryos were fixed at neurula stage for in situ hybridization to detect the PSM marker gene expression: (A--C) PAPC, (D, E) Thy, (F, G) XDelta-1, (H, I) XBra, (J, K) Mespo. Bracket in panels B and E indicates the disruption of segmental expression of PAPC and Thy, respectively. Bracket in panel C indicates the rescue of PAPC expression by coinjecting Mespo MO with pCS–mMespo. Arrowhead in panel G indicates the disruption of segmental expression of XDelta-1. Arrowhead in panel I indicates the increase of XBra expression. Arrowhead in panel K indicates the increase of Mespo expression. |
