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Fig. 5. Expression of Xmmp-9 mRNA associated with corneal wound healing, in various epithelial wounds, and during the earliest stage of limb regeneration following amputation. A: Left superolateral view 24 hr after lens removal. Expression is restricted to the posterior corneal wound. Pigmented spots on the surface of the eye are xanthophores, which normally develop even in many albino embryos. B: Left lateral view showing expression associated with a lateral epithelial wound 24 hr after surgery in a young stage 41 larva. C: Absence of expression in the unoperated control eye of a stage 52 tadpole larva. D: Expression of Xmmp-9 in sham operated specimens 24 hr after incision of the outer cornea epithelium. E: Negative control with no detectable hybridization of sense RNA probe to the wounded cornea 24 hr after incision of the outer corneal epithelium. F: Xmmp-9 expression in the left hindlimb stump 24 hr after distal amputation in a stage 52 tadpole larva. G: Longitudinal section of an amputated stage 52 limb 6 hr after amputation. Xmmp-9 mRNA is present in the apical ectodermal cap (ep) and the underlying mesoderm (ms). H: Absence of expression in the hindlimb on the unop- erated side. cn, cornea; cw, corneal wound; hl, unoperated hindlimb; ln, lens; ls, hindlimb stump; lw, lateral ectodermal wound; uc, uninjured cornea overlying the central pupillary space. Scale bar =500 um in A, 200 um in B. Scale bar in E applies to C and D and equals 100um. Scale bar in G 50um.Scale bar in H applies to F and equals 100um. |
