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Fig. 3. PR130 depletion affects somite organization and tail development. (A) RT- PCR analysis of X. tropicalis total RNA from different developmental stages. Xpr130 is expressed maternally (stage 7) and throughout early development (stages 10, 18, 22, and 30). RT, RT-PCR without reverse transcriptase. ODC1 expression was used as loading control. (A Lower ) Whole-mount ISH for Xpr130 in embryos showing predominant staining of somites from the earliest stage detectable. (B) ISH of tailbud stage embryo with an Xnkd-specific probe. (C) Control embryo shows organized somites in a chevron-like shape as visualized by whole-mount ISH by using XmyoD as a marker (Left). PR130-depleted em- bryos lack chevron-like shaped somites (Right). Arrowheads show somitic borders. (D) Similar to B but without XmyoD. Shown are embryos injected with control MO (CoMO) (Left), PR130 MO (Center), and PR130 MO plus hPR130 RNA (Right). (Lower) Sagittal sections of the somites. |
