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Fig. 6. Spatial distribution of Xp54nrb mRNA during Xenopus early organogenesis. Photomicrographs of whole-mounts and sections through the corresponding whole mounts for stage 23 (A, B) and stage 35 (C-F) embryos are shown. (A) Whole mount and section analysis (B) at stage 23 show Xp54nrb expressed in optic cup (oc), otic vesicle (ov), branchial arches (ba) and mesencephalon (Me) (whole mount, lateral view, anterior is left). (C) Anterior view at stage 35 shows similar labelling patterns to the stage 23 embryo with neuroretina, encephalon and spinal chord labelled. (D) In the transverse section at the trunk level, only the ventricular zone of the spinal cord is stained (white arrowheads). (E) Section at the head level through the eye (F), enlargement of E) the dorsal part of D the spinal chord, and the inner layer of the neuroretina are labelled.
No staining is detected in the lens or in the ciliary marginal zone (CMZ, black arrowhead in F). Abbreviations: ba; branchial arches, cg; cement gland, CMZ, ciliary marginal zone, G, ganglion cell layer, I, inner nuclear cell layer, L, lens, Me; mesencephalon, O, outer nuclear cell layer, ov; otic vesicle, oc; optic cup, Rh; rhombencephalon, RPE retinal pigmented epithelium, Te; telencephalon, Scale bars 0.5 mm (in A-D), and 0.1 mm (in E, F). |
